Changes in sugar, organic acid and free amino acid levels and the expression of genes involved in the primary metabolism of oleocellosis in citrus peels

Abstract

Oleocellosis is a physiological disorder in citrus fruit and causes extensive economic damage due to the surface blemishes it creates. It was reported that oleocellosis always occurs during preharvest maturation and postharvest storage. In the present study, the oleocellosis incidence of Jincheng orange, Navel orange and Ponkan were found to be different during preharvest maturation, however, no differences were found during postharvest storage. Additionally, it was interesting that the outbreak period of oleocellosis incidence was 0–12 d during postharvest storage. Climate change has been reported as a factor promoting oleocellosis development. However, little information is available regarding how primary metabolites and the expression of genes involved in sugar, organic acid and free amino acid metabolism in citrus change to adjust to new environments. Metabolic profiling obtained by gas chromatography-mass spectrometry (GC‒MS) and amino acid analysis showed that the accumulations of fructose, glucose, sucrose, maltose, mannose, citric acid, α-ketoglutarate, 2-keto-d-gluconic acid, glutamate, valine, glycine and threonine might play major roles in adaptation to changes in oleocellosis peels for three types of citrus fruit. However, decreased contents of malic acid, gluconic acid and proline were observed, possibly due to consumption in energy metabolism or reflecting a unique characteristic in this disorder. Regarding gene expression in primary metabolism pathways obtained by high-throughput mRNA sequencing (RNA-Seq) technology, upregulated genes encoding alpha-glucosidase, beta-glucosidase, beta-fructofuranosidase, alpha-amylase, beta-amylase, malate dehydrogenase, CTP synthase (glutamine hydrolysing), serine-glyoxylate transaminase, serine/glycine hydroxymethyltransferase and proline dehydrogenase were the main changes in this disorder.