Abstract
Alterations in contractile force as well as heart sarcolemmal, microsomal, and mitochondrial activities were investigated by perfusing rat hearts with different concentrations (0.5–1.5% (v/v)) of ether for 2 min. Contractile force of the perfused heart was depressed by 0.5–1.0% ether and completely abolished by 1.25–1.5% ether. The contractile force recovered fully upon reperfusion when the hearts were preperfused with 0.5–0.75% ether but the recovery was partial in hearts preperfused with 1.0–1.25% ether. The hearts preperfused with 1.5% ether did not recover their ability to generate contractile force upon reperfusion. The Na+–K+-ATPase activity of sarcolemma obtained from hearts perfused with 0.75–1.0% ether was higher than the control value. The sarcolemmal Na+–K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, calcium binding, and adenylate cyclase activities were decreased in hearts perfused with 1.5% ether and these activities were further decreased upon reperfusion. The microsomal calcium binding and uptake activities were depressed in hearts perfused with 1.0–1.5% ether and these changes were irreversible. The mitochondrial calcium binding and uptake activities as well as oxidative phosphorylation activity were also depressed in hearts perfused with 1.0–1.5% ether; however, irreversible damage was seen in hearts perfused with 1.5% ether. Electron microscopic examination showed occasional vacuolization of mitochondria in hearts perfused with 1% ether and swelling of sarcoplasmic reticulum and mitochondria as well as damage to myofilaments were seen in hearts perfused with 1.5% ether. These results suggest the involvement of alterations in subcellular membrane system in the cardiotoxic actions of ether.
Published Version
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