Abstract
To investigate the regulation of intracellular store-operated calcium channels (SOCCs) in detrusor overactivity (DO) during detrusor function changes in Sprague-Dawley rats. Sixty female Sprague-Dawley rats were randomized into control and DO groups. The contraction of the smooth muscle of the bladder was evaluated invivo using smooth muscle strips. Changes in intracellular calcium ions were observed using confocal microscopy with preload fluo-4 AM, the SOCC agonist cyclopiazonic acid (CPA; 10μM) and inhibitor SKF-96365 (10 μM). Cell currents were recorded with the whole-cell patch-clamp technique. The invitro frequencies of bladder smooth muscle contraction were significantly different (P<.05) between the DO and control groups, and the amplitudes were not significantly different (P >.05). The changes in intracellular calcium ions and current density were significantly different between the 2 groups (P<.05). SOCCs were involved in DO and caused variations in muscle contraction.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
