Abstract
Soybean seedlings were grown at 28°C in the dark or the light for 12 days, and four classes of sterol lipids, sterol esters (SE), free sterols (St), acylated steryl glycosides (ASG) and steryl glycosides (SG), were isolated from the axis and root, respectively. Each sterol lipid (SE, ASG and SG) obtained was hydrolyzed and then divided into sterol, fatty acid and/or sugar fractions. The hydrolysates and St were analyzed mainly by gas-liquid chromatography (GLC).During germination, the amounts of ASG markedly increased, especially in the light-grown seedlings, whereas SE maintained almost constant values. The compositions and changing patterns of the sterol in sterol lipids were similar between the dark-grown and light-grown seedlings, and among epicotyl, hypocotyl and root. The changing patterns of fatty acid composition differed between SE and ASG. SE was found to have high proportions of unsaturated fatty acids, whereas ASG had high proportions of saturated fatty acids. In general, the changes in consti...
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