Abstract
Soluble proteins, peroxidase, esterase, glutamate dehydrogenase and alcohol dehydrogenase isoenzyme patterns from developing grains of CSV-5 and high lysine sorghum (IS 11758) were studied. Isoelectric focussing pattern of soluble proteins from mature seeds of CSV-5 and high lysine sorghum differed both qualitatively and quantitatively. Presence of characteristic proteins with p I value 6.70 to 6.90 in high lysine sorghum and with p I value 5.25 and 7.19 in CSV-5 indicates characteristic nature of these proteins. Soluble proteins from immature kernels also showed differences. Glutamate dehydrogenase patterns were identical. Alcohol dehydrogenase isoenzymes showed differences from high lysine and normal sorghum. Changes in ADH patterns were also evident during development. Esterase patterns at milky and mature stage differed both quantitatively and qualitatively. Differences in isoenzyme patterns of esterase and peroxidase have been observed in mature grains of CSV-5 and high lysine sorghum both by gel electrophoresis and isoelectric focussing.
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