Changes in soil microecology of gangue reclamation areas after 10 years of in situ restoration with herbaceous plants (Artemisia sacrorum and Imperata cylindrica) and trees (Populus spp.)

Abstract

Changes in the soil ecological microenvironment of coal gangue contaminated areas were evaluated after 10 years of in situ restoration with Artemisia sacrorum, Imperata cylindrica, and Populus spp. The changes in soil enzyme activity, ion spectrum, bacterial community structure and function, and metabolic spectrum were analyzed in an unrestored coal gangue pollution area (Control), an Artemisia sacrorum + Imperata cylindrica remediation area (T1), and a Populus remediation area (T2). Phytoremediation significantly increased the soil acid phosphatase activity by 4.62–7.36 times, and decreased the electrical conductivity by 6.2–85.5%. The overall reduction trend for heavy metals in the soil was shallow layer > middle layer > deep layer, with significant reductions seen for Cs, As, Co, Pb, Ni, U, and Cr. The number and diversity of shallow soil microbial communities increased significantly, with Actinobacteria, which participate in the carbon and nitrogen cycles, and Proteobacteria, which can tolerate/metabolize multiple heavy metals, becoming the dominant species in the restoration area. Microbial genes for glycosyltransferase, transcriptional regulators, and toluene degradation changed significantly. Phytoremediation by both remediation strategies significantly changed the metabolic level of lipids and lipid-like molecules in the soil, while significantly enriching the pathways of ABC transporters and galactose metabolism, which are essential for soil detoxification, ion transport, and carbon cycling. Bacteria such as Thiobacillus and Pseudarthrobacter were important drivers of soil metabolism. In general, the in situ phytoremediation of soil contaminated by coal gangue improved the overall soil ecological microenvironment.