Changes in small molecular weight biomarkers identified by NMR spectroscopy in response to dietary treatment with two conjugated linoleic acid isomers (c9,t11; t10,c12) in a murine collagen‐induced arthritis model

Abstract

Dietary conjugated linoleic acid (CLA) has been shown to have anti‐inflammatory properties in a mouse model of collagen‐induced arthritis. After 42 days of dietary treatment, mice fed 0.5% c9,t11 or t10,c12 CLA exhibited reductions in clinical arthritic score (29% and 41% respectively) and paw swelling (36% and 73% respectively) compared to corn oil fed arthritic mice. The objective was to measure changes in metabolic responses to chronic arthritic inflammation while determining how the anti‐inflammatory properties of dietary c9,t11 and t10,c12 CLA might improve or restore metabolic processes near normalcy as reporters of improved clinical signs of arthritis. 1H nuclear magnetic resonance spectroscopy was utilized to profile changes in plasma metabolites between each treatment of arthritic and non‐arthritic mice. Total plasma amino acid levels (TAA) were significantly lower (33%, p<0.05) in arthritic corn oil fed mice compared to non‐arthritic mice in response to chronic inflammation. Although both CLA isomer treatments increased TAA in arthritic mice, only t10,c12 CLA restored TAA to normalcy of non‐arthritic mice. The improvement in plasma TAA in response to dietary treatment with c9,t11 or t10,c12 CLA reflect the intensity of each CLA isomer's anti‐inflammatory actions in clinical arthritic score and paw swelling.This research was supported by a Hatch grant (142‐PRJ16AU‐4).Grant Funding Source: Hatch grant (142‐PRJ16AU‐4)