Changes in sleep–wakefulness in the medial preoptic area lesioned rats: role of thermal preference

Abstract

Changes in sleep–wakefulness (S–W) were studied in adult male Wistar rats, along with body temperature ( T b), locomotor activity (LMA) and thermal preference, after the lesion of the medial preoptic area (mPOA) with N-methyl-D-aspartic acid (NMDA). The sleep was decreased after the lesion of the mPOA, but there was recovery when the rats were given freedom to stay in an ambient temperature ( T amb) which they preferred. When given a choice between three T amb (24, 27 and 30 °C), the rats preferred 27 °C before the mPOA lesion, and 24 °C during the initial days after the lesion. There was a shift in the thermal preference to 30 °C, on the fourth week after the lesion, which coincided with the considerable recovery of sleep. The preference for higher T amb probably helped to improve sleep, as T amb of 30 °C is known to promote sleep. When the lesioned rats were not given the freedom to select the T amb, there was no recovery in sleep. The mPOA seems to be essential for increasing the durations of slow wave sleep (SWS) episodes, especially the light SWS (S1), as they remained shorter than the pre-lesion value, even when the rats were given freedom to stay in a preferred T amb. The homeostatic recovery of sleep, especially the night time sleep, resulted in the disruption of circadian sleep rhythm. But, the LMA, T b and thermal preference maintained their diurnal variation. T b and LMA were elevated after the mPOA lesion and they remained so till the end of the study.