Changes in serum sex steroid levels throughout the reproductive cycle of Bufo arenarum females

Abstract

The changes in the serum levels of the sexual steroids estradiol-17β (E 2), testosterone (T), dihydrotestosterone (DHT), and progesterone (P) in Bufo arenarum females were determined by radioimmunoassay (RIA) during 3 consecutive cycles (1999–2001). The serum concentrations of T and DHT, which showed a close parallelism during the annual reproductive cycle, exhibited the highest levels during the preovulatory period, when oogenesis is advanced, while lowest serum levels of these hormones were found during the ovulatory period. The data obtained for E 2 showed a pattern contrary to that determined for androgens. The maximum E 2 concentrations detected in the early postovulatory period might be associated with vitellogenesis and follicular growth. Lowest E 2 concentrations were reached during the period in which B. arenarum undergoes its final hibernation stage. Serum P showed a peak during the preovulatoy period, related to the induction of nuclear maturation in full grown oocytes. A strong decrease in the levels of the circulating hormones was observed after ovariectomy. Our results showed that, out of the four hormones examined, T and DHT were the best indicators of ovarian and oviductal stage, as shown by the strong positive correlation found between androgen levels and organ weight, while E 2 showed a weak negative correlation with ovarian and oviductal weight.