Changes in Prothrombin Time and Platelet Parameters of Mice Treated with Vitamins C and E

Hongwei Li,Wei Wang,Qinglu Wang,Jing Zhang,Xinzhuo Tu,Xiangcui Chen,Tongbing Qi,Jing Cheng,Weitong Cui

doi:10.3844/ajbbsp.2020.170.176

Abstract

This study aims to elucidate the influence of supplementation with different doses of Vitamin C (VC) and Vitamin E (VE) on Prothrombin Time (PT) in mice; forty eight mice are randomly divided into six treatment groups, which are fed different dose VC and VE except for control Check (CK) for eight weeks. Blood samples are obtained from the mouse canthus to measure PT and platelet parameters. Administration of different doses of VC and VE to mice cause coagulation function damage.

Highlights

Under normal circumstances, the functions of blood coagulation, anticoagulation, platelet and fibrinolysis in peripheral blood are in a state of dynamic equilibrium, the coagulation mechanism is hyperactive and the anticoagulant mechanism is relatively weakened when this balance is broken
Under a large number of oxidative free radical condition, endothelial cells injury can cause by inducing oxidative stress injury, endothelial cells release Tissue Factor (TF), TF can trigger endogenous and exogenous coagulation mechanisms Endothelin (ET) and other substances cause vasoconstriction and VW factor causes blood platelet aggregation (Chen, 2005)
Platelet hematocrit (PCT) first increased and decreased and the Vitamin C (VC) control group had the highest PCT among all groups

Summary

Introduction

The functions of blood coagulation, anticoagulation, platelet and fibrinolysis in peripheral blood are in a state of dynamic equilibrium, the coagulation mechanism is hyperactive and the anticoagulant mechanism is relatively weakened when this balance is broken. There are many infuence factors of coagulation function, one of which is considered the imbalance of oxidation and anti-oxidation in the body. Under a large number of oxidative free radical condition, endothelial cells injury can cause by inducing oxidative stress injury, endothelial cells release Tissue Factor (TF), TF can trigger endogenous and exogenous coagulation mechanisms Endothelin (ET) and other substances cause vasoconstriction and VW factor causes blood platelet aggregation (Chen, 2005). Platelet count (PLT), platelet parameters including Mean Platelet Volume (MPV) and platelet hematocrit (PCT) are significantly lower than those of healthy individuals. PT and platelet parameters are used as important clinical reference indicators (Martí-Carvajal and Solà, 2012)

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Conclusion