Abstract
The effects of heat treatment on the changes in the protein components and size distribution of bovine milk fat globules were investigated. The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that some membrane proteins, such as xanthine oxidase and butyrophilin, had disappeared or were weakly stained in the gel due to heating of milk. The volume surface average diameter of raw milk fat globules ranged from 1.15 to 7.42 μm. When the heating temperature was above 90 °C or the heating time was prolonged above 10 min, the average diameters of heated milk fat globules increased significantly (P < 0.05).
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