Changes in Polyphosphoinositide Metabolism in Globodera Rostochiensis Following Stimulation To Hatch By Potato Root Diffusate

Abstract

Eggs of Globodera rostochiensis were incubated for 2.5 h in 13.3 mM myo-[2- 3 H] inositol to allow incorporation of the labelled compound into phosphoinositides. The subsequent in vivo metabolism of these phospholipids released the water soluble products glycerophosphoinositol, (GPIns), inositol 1-phosphate (IP1); inositol 1,4-biphosphate (IP2) and inositol 1,4,5-triphosphate (IP3) which were separated by ion exchange chromatography before the radiolabel incorporated into each was quantified using a scintillation counter. Exposure of eggs to labelled myo-inositol in either active or heat-inactivated hatching factor in potato root diffusate resulted in a significantly higher recovery of the three inositol phosphates from individuals receiving the stimulus to hatch. The results substantiate earlier work indicating a rapid initial action of hatching factor on unhatched juveniles of G. rostochiensis.