Abstract
The metabolism of platelets in contact with poly(acrylamide-co-methacrylic acid) (PAAmMAc) particles was evaluated in terms of changes in cytoplasmic free calcium levels ([Ca2+]i), aderosine triphosphate (ATP) release, and membrane fluidity. PAAmMAc particles induced little increase in [Ca2+]i in platelets. However, PAAmMAc particle-treated platelets demonstrated a smaller response to thrombin, to calcium ionophore A23187, and to polystyrene (PSt) particles, suggesting that PAAmMAc surfaces may regulate [Ca2+]i and ATP release by altering platelet metabolism. Sodium azide-treated platelets showed an increase in [Ca2+]i when contacting PAAmMAc particles, supporting the idea that PAAmMAc surfaces could regulate platelet function. Fluorescence polarization measurements revealed that PAAmMAc particles increased membrane fluidity in platelets, which effect may be due to physicochemical interaction with PAAmMAc surfaces.
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