Changes in plasma glucagon, insulin and tissue metabolites associated with prolonged fasting in brown trout ( Salmo trutta fario) during two different seasons of the year

Abstract

1. 1. Pyrenean brown trout juveniles ( Salmo trutta fario) were fasted for 50 days in late winter (experiment 1) and summer (experiment 2). Plasma insulin, glucagon and glucose and some metabolites in plasma and in tissues were analysed. 2. 2. Glucagon increased significantly on the 3rd day of fasting in the winter experiment (controls 653.7 ± 92.4 pg/ml, fasted 912.7 ± 135.2 pg/ml), and the same tendency was observed on the 5th day in the summer experiment (controls 430.5 ± 56.2 pg/ml, fasted 555.5 ± 95.3 pg/ml). During this initial period of fasting, plasma glucose was maintained in both experiments (75.5 ± 4.7–67.6 ±4.1 mg/100ml), but from day 8, glucose and glucagon decreased simultaneously. 3. 3. Insulin decreased from the beginning of fasting, reaching lowest values after 50 days of fasting (winter experiment: controls 6.4 ± 0.3 ng/ml, fasted 1.6 ± 0.1 ng/ml; summer experiment: controls 4.8 ± 0.1 ng/ml, fasted 1.2 ± 0.2 ng/ml). Glucagon/insulin molar ratio (G/I) increased after 3 days in the winter experiment (controls 0.21 ± 0.02, fasted 0.39 ± 0.05), while in the summer experiment, the ratio rose from day 5 and reached a peak at day 30 (controls 0.16 ± 0.02, fasted 0.48 ± 0.07). 4. 4. Muscle proteins were significantly mobilized after 50 days of fasting. Visceral index decreased significantly after day 15 while liver glycogen was already significantly lower at day 8. However, in the summer experiment, a transitory increase of liver glycogen was observed at day 30, coincicling with the peak of G/I.