Changes in phosphatases activities in uterine secretion during estrous cycle:A possible indicator of important biochemical changes in buffaloes uterine microenvironment

Abstract

Defining biochemical nature of uterine secretion has been the focus of recent research interest due to conception failure after embryo transfer and normal pregnancy in ruminants including buffaloes. Proteins are important as biomolecules for cell function, and phosphatases control the protein function. Therefore identification of changes on secretory proteins and phosphatases activities during early luteal, mid luteal, late luteal and follicular stages of estrous cycle in buffaloes is attempted. The luteal stages uterine secretion contained significantly more protein as compared to the follicular stages of cycle. Two-fold increase in specific and total activities of acid phosphatases during mid-luteal phase and 10-fold specific and 100-fold increase in total alkaline phosphatases activities during late luteal and follicular stages was observed as compared to other stages of the cycle. Corpus luteum growth and regression did not influence the total uterine protein secretion or phosphatases activities. The changes of phosphatases in buffalo uterine secretion during different stages of estrous cycle might be the indicator of the process of endometrial epithelial differentiation and degeneration.