Changes in ovarian development and plasma levels of sex steroid hormones in the wild female Japanese sardine (Sardinops melanostictus) during the spawning period

Abstract

In order to clarify the spawning pattern of Japaneses sardine (Sardinops melanostictus) during the spawning period, changes in ovarian histology, frequency distributions of oocyte diameter and plasma levels of oestradiol-17β (E2) and 17a,20β-dihydroxy-4-pregnen-3-one (17a,20β-diOH-P) were examined in female fish captured in the region off Kyushu and Shikoku in Japan. With the development of the ovary, the gonadosomatic index (GSI) increased gradually and the size of oocytes became larger. When the GSI exceeded 13, the translucent hydrated eggs began to dominate and ovulation subsequently occurred. After ovulation, GSI decreased to less than 3. Post-ovulatory follicles were found only in the lower GSI ranges and atretic oocytes were always observed in the ovary. Plasma E2 levels increased along with ovarian development but decreased after completion of yolk accumulation. On the other hand, 17a,20β-diOH-P showed the highest level just before and after ovulation. Ovarian histology and hormonal profiles suggest that the sardine spawns repeatedly during the spawning period. Based on the concentration of 17a,20β-diOH-P, the female Japanese sardine was estimated to ovulate before the middle of the night.