Abstract
Objective To observe the changes in open probability and protein expression of large conductance Ca2+-activated K+ (BK) channel in retinal vascular smooth muscle cells (RVSMCs) of diabetic rats. Methods Streptozotocin (STZ)-induced rat diabetic animal model was established by STZ injection intraperitoneally.RVSMCs were isolated by enzyme digestion. The BK currents in control and diabetic groups were recorded by patch clamp technique in single channel configuration. BK channel protein expression in control and diabetic group were measured by Western blot. Results Compared with control group, the NP0 of BK channels in diabetic group were significantly increased (t=4.260, P 0.05); however, β1-subunit protein expression was remarkably increased in diabetic group (t=5.146, P<0.05). Conclusion The NP0 of BK channels and β1-subunit protein expression are increased in RVSMCs of diabetic rats. Key words: Diabetic retinopathy/etiology; Retinal artery; Potassium channels; Patch-clamp techniques; Animal experimentation
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