Abstract
We used a rat model of whole thorax x-ray irradiation to profile the microRNA (miRNA) in lung and blood up to 4 weeks after radiation. MiRNA from normal and irradiated Wistar rat lungs and whole blood were analyzed by next-generation sequencing and the changes by radiation were identified by differential deRNA-seq 1, 2, 3 and 4 weeks after irradiation. The average total reads/library was 2,703,137 with a mean of 88% mapping to the rat genome. Detailed profiles of 100 of the most abundant miRNA in rat blood and lung are described. We identified upregulation of 4 miRNA, miR-144-5p, miR-144-3p, miR-142-5p and miR-19a-3p in rat blood 2 weeks after radiation that have not previously been shown to be altered after radiation to the lung. Ingenuity Pathway Analysis identified signaling of inflammatory response pathways. These findings will support development of early detection methods, as well as mechanism(s) of injury and mitigation in patients after radiotherapy or radiological accidents.
Highlights
We used a rat model of whole thorax x-ray irradiation to profile the microRNA in lung and blood up to 4 weeks after radiation
Radiation-induced lung injury is characterized by acute pneumonitis and chronic fibrosis[1,2,3] both of which can be lethal
We have developed a rat model of whole thoracic radiation by X-rays to induce pneumonitis from 6–12 weeks post exposure and pulmonary fibrosis after 30 weeks[4,5]
Summary
We used a rat model of whole thorax x-ray irradiation to profile the microRNA (miRNA) in lung and blood up to 4 weeks after radiation. Ingenuity Pathway Analysis identified signaling of inflammatory response pathways These findings will support development of early detection methods, as well as mechanism(s) of injury and mitigation in patients after radiotherapy or radiological accidents. We have developed a rat model of whole thoracic radiation by X-rays to induce pneumonitis from 6–12 weeks post exposure and pulmonary fibrosis after 30 weeks[4,5]. MiRNA expression was studied using the nanostring nCounter multiplex platform, which is capable of detecting approximately 600 mouse specific miRNAs. Recently Xie et al reported lung miRNA expression in response to radiation-induced lung injury in rats.
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