Changes in miRNA expression profile between stress-vulnerable and stress-resilient rats in Chronic Mild Stress (CMS) - an animal model of depression.

Abstract

Event Abstract Back to Event Changes in miRNA expression profile between stress-vulnerable and stress-resilient rats in Chronic Mild Stress (CMS) - an animal model of depression. Dariusz Zurawek1*, Agata Faron-Gorecka1, Maciej Kusmider1, Magdalena Kolasa1, Paulina Pabian1, Joanna Solich1, Kinga Szafran1, Piotr Gruca2, Mariusz Papp3 and Marta Dziedzicka-Wasylewska1 1 Institute of Pharmacology Polish Academy of Sciences, Department of Pharmacology Laboratory of Biochemical Pharmacology, Poland 2 Institute of Pharmacology Polish Academy of Sciences , Department of Pharmacology Laboratory of Behavioural Pharmacology, Poland 3 Institute of Pharmacology Polish Academy of Sciences, Department of Pharmacology Laboratory of Behavioural Pharmacology, Poland Prolonged, chronic stressful events are considered to be one of the major causes of depression, however many people and animals exposed to stress seem to be resilient. Only a few studies have focused on stress resilience phenomenon. Depression may result from impairment in neuronal plasticity which may be associated with altered miRNA expression level - a class of newly discovered posttranscriptional regulators of gene expression. We used Chronic Mild Stress (CMS) paradigm which is well characterised animal model of depression. We observed that in this model about 30% of rats exposed to chronic, stressful conditions demonstrate resilience to stress what is manifested as not altered consumption of a palatable 1% sucrose solution by this group of animals. We investigated a global miRNA expression profile in serum of rats subjected to chronic mild stress to identify the potential, non-invasive molecular markers differentiating animals that demonstrated resilience to stress from stress-reactive ones. After two weeks of CMS procedure we identified three groups of rats based on their sucrose consumption: control (12,1±0,6g), anhedonic (5,3±0,4g, p<0,0001) and stress resilient (11,6±0,4g,). Animals were sacrificed and the serum was collected, from which miRNA was purified. The RNA concentration and quality was measured with NanoDrop spectrophotometer. RT-PCR based TaqMan Array Rodent MicroRNA Cards were used to screening the changes in miRNA expression induced by stress conditions and response to stress. Using Expression Suite Software analysis we found that stress significantly altered the expression of one miRNA in anhedonic rats as compared to control group, while in stress-resilient animals the expression of 16 miRNAs was changed. This data indicate that resilient animals can actively cope with stressful conditions leading to "žstress-resistant" phenotype. miRNAs may play important role in stress resilience by pleiotropic modulation of expression of many genes. The study was supported by National Science Center Grant UMO-2012/07/B/NZ7/01164, Poland. Keywords: Depression, miRNA, Animal Models, Chronic mild stress, stress-resilience Conference: XII International Conference on Cognitive Neuroscience (ICON-XII), Brisbane, Queensland, Australia, 27 Jul - 31 Jul, 2014. Presentation Type: Poster Topic: Emotional and Social Processes Citation: Zurawek D, Faron-Gorecka A, Kusmider M, Kolasa M, Pabian P, Solich J, Szafran K, Gruca P, Papp M and Dziedzicka-Wasylewska M (2015). Changes in miRNA expression profile between stress-vulnerable and stress-resilient rats in Chronic Mild Stress (CMS) - an animal model of depression.. Conference Abstract: XII International Conference on Cognitive Neuroscience (ICON-XII). doi: 10.3389/conf.fnhum.2015.217.00376 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Feb 2015; Published Online: 24 Apr 2015. * Correspondence: Mr. Dariusz Zurawek, Institute of Pharmacology Polish Academy of Sciences, Department of Pharmacology Laboratory of Biochemical Pharmacology, Krakow, Poland, zurawek@if-pan.krakow.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Dariusz Zurawek Agata Faron-Gorecka Maciej Kusmider Magdalena Kolasa Paulina Pabian Joanna Solich Kinga Szafran Piotr Gruca Mariusz Papp Marta Dziedzicka-Wasylewska Google Dariusz Zurawek Agata Faron-Gorecka Maciej Kusmider Magdalena Kolasa Paulina Pabian Joanna Solich Kinga Szafran Piotr Gruca Mariusz Papp Marta Dziedzicka-Wasylewska Google Scholar Dariusz Zurawek Agata Faron-Gorecka Maciej Kusmider Magdalena Kolasa Paulina Pabian Joanna Solich Kinga Szafran Piotr Gruca Mariusz Papp Marta Dziedzicka-Wasylewska PubMed Dariusz Zurawek Agata Faron-Gorecka Maciej Kusmider Magdalena Kolasa Paulina Pabian Joanna Solich Kinga Szafran Piotr Gruca Mariusz Papp Marta Dziedzicka-Wasylewska Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.