Abstract
Low-temperature storage is the primary postharvest method employed to maintain fruit quality and commercial value. However, pitting can develop during refrigeration, especially during the shelf life. In this study, a membrane lipidomic approach was employed to analyze the potential relationship between pitting and membrane lipid metabolism during post-cold-storage shelf life. We also determined the changes in ultrastructure and water distribution by low-field nuclear magnetic resonance (LF-NMR) and assessed the permeability of membrane, membrane lipid peroxidation, proline and malondialdehyde contents, and the activity and gene expression of phospholipase D and lipoxygenase, which are involved in membrane lipid metabolism. The results indicated that the changes in blueberry phospholipids during storage could be caused by cold stress. Furthermore, dehydration is a manifestation of chilling injury. Finally, the significant increase in electrolyte leakage, content of malondialdehyde and proline, and activity of phospholipase D and lipoxygenase in chilled blueberry also indicated that membrane lipid metabolism plays an important role in cold stress response.
Highlights
Blueberry (Vaccinium spp.) is popular among consumers and it has high antioxidant activity and health benefits, as it can prevent diseases (Li et al, 2012; Gupta et al, 2015; Chu et al, 2018)
Tissue damage or death suffered at temperatures above freezing but below 15◦C, i.e., chilling injury (CI) (Wolfe, 2010) in fresh agricultural products at low temperature storage has limited the commercial value of several fruits, including Nanguo pear (Shi et al, 2017), loquat (Cao et al, 2011), sweet pepper (Wang et al, 2016), and pineapple (Nukuntornprakit et al, 2015), among others
The changes in blueberry phospholipids during cold storage could be caused by cold stress
Summary
Blueberry (Vaccinium spp.) is popular among consumers and it has high antioxidant activity and health benefits, as it can prevent diseases (Li et al, 2012; Gupta et al, 2015; Chu et al, 2018). Tissue damage or death suffered at temperatures above freezing but below 15◦C, i.e., chilling injury (CI) (Wolfe, 2010) in fresh agricultural products at low temperature storage has limited the commercial value of several fruits, including Nanguo pear (Shi et al, 2017), loquat (Cao et al, 2011), sweet pepper (Wang et al, 2016), and pineapple (Nukuntornprakit et al, 2015), among others. At the early stage of refrigeration, the physiological characteristics of the cell membrane of Chilling Injury in Blueberry fruit and vegetable tissues will change; with the prolongation of refrigeration and transfer to room temperature storage, membrane lipid components will further change and the accumulation of membrane lipid degradation products in fruit and vegetable leads to irreversible membrane lateral phase separation. The fruits show a series of CI symptoms (Marangoni et al, 1996)
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