Abstract
The lipids bound to the I-Z-I fraction and the Myofibrillar Fragmentation Index (MFI) of postmortem ovine Longissimus dorsi stored at 4C were investigated. At zero time of storage total lipids were composed of about 50% neutral lipid and 50% phospholipid. During storage, the neutral lipid fraction showed a significant decrease (P < 0,01) in the relative percentage of triglycerides and a significant increase (P < 0.01) in free fatty adds. The composition ofphospholipids extracted from I-Z-I with chloroform:methanol 2:1 (v/v) remained unchanged. Further treatment of the I-Z-I fraction with chloroform:methanol (2:1, v/v) containing 0.25% (v/v) of concentrated HCl extracted an additional amount of phospholipids. The content of phospholipid extractable from the I-Z-I fraction with acidified organic solvents significantly decreased (P < 0.01) during storage and this change was accompanied by a significant increase (P < 0.01) in the MFI.
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