Abstract
Changes in genetic expression play a central role during the differentiation of intestinal epithelial cells (IEC). Previously we have reported that Jak3 plays an essential role during mucosal wound repair an important ramification for patients with ulcerative colitis and Crohn's disease. Here we determine the physiological significance of Jak‐3 expression during enterocytic differentiation. Jak‐3 expression was determined in sub‐confluent, confluent, 2‐ and 3‐week post‐confluent HT‐29 Cl‐19a cells through western analysis using Jak‐3 antibody. Reduced Jak‐3 expression was observed in sub‐confluent cells and the expression was increased with differentiation. Interestingly an additional lower mol. wt. Jak3 was detected only at 2 weeks of post confluent cells but not at 3 weeks post confluence indicating the possibility of a non phosphorylated form. To determine the effect of Jak‐3 on the differentiation of HT‐29, Jak‐3 was over expressed in HT‐29 cells and its differentiation was monitored using the differentiation marker sucrose isomaltase. Results show that over expression of Jak‐3 potentiate the differentiation of IEC. Densitometric analysis of the ratio of Jak3 to â‐actin expression shows a 1.5 fold increase in Jak3 expression. Taken together these results indicate that Jak3 expression regulate the differentiation of IEC. *This work was supported a grant from CCFA (to NK)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.