Abstract
The prevention of the symplastic entry of metabolites into bud cells may be indicative of dormancy status. Intracellular measurement allows, at least in part, quantification of the sink strength for nutrients of different studied tissues. The aim of this research was to study the changes in intracellular pH (pHi) in vegetative and flower buds of apricot. The pHi measurement, based on DMO (5,5 dimethyloxazolidine 2,4 dione) radioactive probe accumulation, was carried out periodically from December to February on buds collected from mixed twigs of San Castrese cultivar. The vegetative and flower buds and underlying parenchymatic tissues were located on the same node but in different combinations. The flower buds presented significant pHi changes in relation to the end of endo-dormancy and the evolution of phenological stages. In particular, when flower buds were still in deep dormancy, it was possible to record an increase of pHi in the flower primordia tissues just before the first sign of growth reactivation.
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