Changes in ide Leuciscus idus (L.) females’ reproductive parameters after stimulation with carp pituitary homogenate (CPH) and Ovopel: the effect of domestication?

Mirosław Cieśla,Wojciech Andrzejewski,Robert Jończyk,Dariusz Gozdowski,Jacek Rechulicz,Jerzy Śliwiński

doi:10.1007/s10499-013-9668-z

Abstract

Three groups (wild—WS, semi-pond—SPS, and pond-cultured—PS) of ide Leuciscus idus (L.) were examined. Each group consisted of 30 females and 10 males, aged 5-8 years. All groups were stimulated with two agents: carp pituitary homogenate (CPH) and a synthetic hormonal preparation Ovopel. The amount of ovulating females decreased in conjunction with females' domestication level, mainly because of very low efficacy of CPH for pond-reared ide stimulation. The percentage of ovulating females stimulated with Ovopel (97.0 %) was significantly higher comparing to CPH (63.0 %). No significant differences were found in latency time between the groups but spawning synchronization increased in conjunction with ide domestication. The positive correlation between domestication level and latency time, i.e., the higher the domestication level, the higher the latency time, was stated. No significant differences were found in PGSI as well as in total volume of obtained eggs, but groups differed significantly in the number of dry eggs and in number of swollen egg. Significant negative correlation between domestication level and number of swollen eggs, i.e., the higher the domestication level, the lower the number of eggs, was detected. The survival rate at the eyed stage was the highest in WS group (60.4 %) and PS group (57.7 %), and was significantly higher than in SPS group (51.8 %). Eggs obtained after CPH stimulation had higher survival rate at the eyed-egg stage

Highlights

Rapid advancement in human civilization, in the nineteenth and twentieth centuries, caused serious alterations and damage to the natural environment, especially in fish communities inhabiting river ecosystems
No significant differences were found in latency time between the groups but spawning synchronization increased in conjunction with ide domestication
The aim of this paper is to show differences in reproductive parameters of ide females raised in different environments before artificial stripping and stimulation with carp pituitary homogenate or Ovopel

Summary

Introduction

Rapid advancement in human civilization, in the nineteenth and twentieth centuries, caused serious alterations and damage to the natural environment, especially in fish communities inhabiting river ecosystems This statement refers to large migratory fish of Acipenseridae (Gershanovich and Burtsev 1993; Ivanov and Maznik 1997) or Salmonidae families (Solomon et al 1999; Wisniewolski 2002; Thorstad et al 2008) and to smaller and ‘non-migrating’ species like reophilic cyprinids (Lelek 1987; Lusk 1995; Fredrich 2003; Winter and Fredrich 2003). Restocking is one of the most important and popular ways of enhancing fish populations for both fisheries and ecological purposes (Cowx 1994; Bolland et al 2008) Such programs, require numerous and genetically diversified broodstock herds (Philippart et al 1989; Willot et al 1997; Pelczarski 1998). Carp pituitary homogenate (CPH) was used for artificial ide reproduction (Kucharczyk et al 1999; Ciesla et al 2000); since late 90s, alternative agents, such as human chorionic gonadotrophin (hCG) (Kucharczyk et al 1999), synthetic hormonal preparations containing LH–RH-a or GnRH analogues with dopamine antagonist, like Ovopel or Ovaprim, have been implemented in hatchery practice (Targonska-Dietrich et al 2004; Jamroz et al 2008; Kayim et al 2010; Cejko et al 2012)

Objectives

Methods

Results

Conclusion