Changes in hydrogen peroxide homeostasis and cytokinin levels contribute to the regulation of shade-induced senescence in wheat leaves

Abstract

In a previous work we demonstrated that the suppression of blue light in shaded leaves of wheat increases their senescence rate and the development of oxidative stress symptoms. In order to better understand the interaction between the oxidative metabolism and light spectral quality in the regulation of leaf senescence, we studied the evolution of H 2O 2 concentration, protein oxidation, proteolytic activity and cytokinin content in excised leaves, either illuminated (control, “C”) or shaded under blue (“B”, high blue light transmission) or green (“G”, very low blue light transmission) light filters. H 2O 2 concentration significantly increased during the first 9 h after treatment initiation, an effect that was consistently higher in treatments B and C. Leaves from these treatments showed lower chlorophyll and protein degradation rates, lower concentration of oxidized proteins, and maintained higher levels of the cytokinin isopentenyl-adenosine than those from treatment G. When moderate H 2O 2 concentrations were supplied during 6–9 h after the onset of the shade treatments, senescence rate in treatment G was delayed, while the opposite effect was observed in the presence of the H 2O 2 scavengers catalase and, to a lesser extent, dimethylthiourea. These effects were accompanied by an increment or a decrement, respectively, of catalase activity, suggesting that the early changes in H 2O 2 homeostasis in leaves from treatments B and C may contribute to the prevention rather than to the induction of further oxidative damage. Altogether our results show that the suppression of blue light transmission in shaded leaves act as a stress signal that increases their sensitivity to oxidative stress and accelerates cell death.