Abstract
Aim:A good skin graft histopathology is followed by formation of hair follicle, sweat gland, sebaceous gland, blood vessel, lightly dense connective tissue, epidermis, and dermis layer. This research aimed to observe histopathology feature and cytokeratin AE1/AE3 expression on cat skin post skin grafting within a different period of time.Materials and Methods:Nine male Indonesian local cats aged 1-2 years old weighing 3-4 kg were separated into three groups. First surgery created defect wound of 2 cm × 2 cm in size to whole groups. The wounds were left alone for several days, differing in interval between each group, respectively: Group I (for 2 days), Group II (for 4 days), and Group III (for 6 days). The second surgery was done to each group which harvested skin of thoracic area and applied it on recipient wound bed. On day 24th post skin graft was an examination of histopathology and cytokeratin AE1/AE3 immunohistochemistry.Results:Group I donor skin’s epidermis layer had not formed completely whereas epidermis of donor skin of Groups II and III had completely formed. In all group hair follicle, sweat gland, sebaceous gland, and neovascularization were found. The density of connective tissue in Group I was very solid than other groups. Cytokeratin AE1/AE3 expression was found on donor skin’s epithelial cell in epidermis and dermis layer with very brown intensity for Group II, brown intensity for Group II, and lightly brown for Group I.Conclusion:Histopathological structure and cytokeratin AE1/AE3 expression post skin graft are better in Groups II and III compared to Group I.
Highlights
Skin transplant is a transfer of skin tissue from healthy body part to be placed on wounded site
On day 24th post skin graft was an examination of histopathology and cytokeratin AE1/AE3 immunohistochemistry
The density of connective tissue in Group I was very solid than other groups
Summary
Skin transplant is a transfer of skin tissue from healthy body part to be placed on wounded site. Histopathology of wound recovery begins from cell regeneration until organ function return. Donor skin for skin graft has no blood vessel connection with wound bed. This blood vessel at the base of the wound must heal rapidly and develop so that donor skin can fuse with recipient skin. If blood vessels do not grow and develop well, donor skin will undergo necrosis [1]. Allograft and xenograft method of skin transplant have often been described in small animal [3]. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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