Changes in histone H3 and H4 multi-acetylation during natural and forced dormancy break in potato tubers.

Abstract

The effects of post-harvest storage and dormancy progression on histone acetylation patterns were examined in potato (Solanum tuberosum L. cv. Russet Burbank) tubers. Storage of field-grown tubers at 3 degrees C in the dark resulted in the progressive loss of tuber meristem dormancy, defined as measurable growth after transfer to 20 degrees C for 7 days. Dormancy emergence was concomitant with sustained increases in histone H3.1 and H3.2 multi-acetylation, and with transient increases in H4 multi-acetylation that peaked 4-5 months post-harvest. Treatment of dormant tubers with bromoethane (BE) resulted in rapid loss of dormancy over 9 days. Similar to cold-stored field-grown tubers, dormancy break in BE-treated tubers occurred at the same time as transient rises in H4 and H3.1/3.2 multi-acetylation, peaking at days 1 and 4, respectively. BE treatment also resulted in small increases in RNA synthesis at day 6, and a three-fold, sustained activation of DNA synthesis thereafter. A defined sequence of epigenetic events, beginning with previously characterized transient cytosine demethylation, followed by increased H3 and H4 histone acetylation and ultimately, tuber meristem re-activation, may thus exist in potatoes during dormancy exit and resumption of rapid growth.