Changes in henna (Lawsonia inermis L.) morphological traits under different deficit irrigations in the southern Tunisia

Abstract

Henna plant belongs to continental oases where water shortage constitutes the essential limiting factors of its agricultural production. Lawsonia inermis L. (Lytraceae) is often exposed to severe drought stress in Gabes; a Tunisian arid region. The present study was carried out to evaluate the impact of water stress on the morphology of Tunisian henna plants. Thus, an experiment of four months was carried out under greenhouse at the Institute of Arid Region in Medenine, Tunisia. Henna was exposed to three different irrigation regimes, whereby the plants where irrigated to field capacity (control, T0), 50% of the control (moderate stress, T1) and 25% of the control (severe deficit irrigation, T2). Results showed that, leaf area (LA), leaf number and stem length of henna, decreased in response to the studied stress. The effect of water stress was clearly observed on those parameters. Moderate drought (T1) did not damage henna morphology, and the plants grew better than without water limitation (T0). Furthermore, the water stress-typical responses were shown as time and severity dependent in all the measured parameters. Indeed, lowest water availability treatment (T2) induced significant decrease in total number of leaves, as well as reductions in LA. Under this severe water stress (T2); LA was reduced by 65.79%, compared to control, at 60 days after the initiation of the bioassay. Stem length decreased significantly in the most severe water stress, this reduction was about 44%. Globally, we conclude that henna plant growth decreased progressively to long-term water limitation.