Abstract
AbstractLeaves of Quercus robur L. were infected with conidia of Microsphaera alphitoides Griffon and Maublanc. Crude enzyme extracts of healthy and infected tissue, taken during a 7‐day experimental period, were assayed, using spectrophotometric and manometric techniques, for glycolate oxidase, NAD‐ and NADP‐glyoxylate reductase activities. After 2 days, extracts from infected tissue contained significantly higher activities of glycolate oxidase and NAD‐glyoxylate reductase than extracts from controls, but the activity of NADP‐glyoxylate reductase, after an initial rise, was significantly reduced below that in control extracts. The reduction in glycolate oxidase activity may be partly due to a deficiency in flavin mononucleotide in infected tissue. A possible connection between the reduction in flavoprotein enzyme activity, photorespiration and susceptibility to infection is described.
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