Changes in gene expression of adipose tissue CD14+ cells in patients with Type 2 diabetes mellitus and their relationship with environmental factors.

Abstract

To study the gene expression of adipose tissue CD14+ cells in patients with Type 2 diabetes mellitus (T2DM) based on chip data, screen differentially expressed genes, and analyze their relationship with the environmental factors. The data of GSE54350 were obtained from the public database of gene expression profiling. The data were pre-processed by Network Analyst, String 11.0, Cytoscape 3.7.1, and other analytical software. The differentially expressed genes were analyzed by gene ontology biological function and kyoto encycopedia of genes and genomes (KEGG) signaling pathway to establish differential gene protein interaction network, transcription factor-gene regulatory network, microRNA-gene regulatory network, environmental factors-gene regulatory network, and other interaction systems. The gene expression pattern of CD14+ cells in adipose tissue of obese T2DM patients was significantly different from that of obese non-T2DM patients. There were 19 differentially expressed genes with up-regulation. The differentially expressed genes were mainly involved in ARP2/3 complex regulation of actin cytoskeleton, positively associated with biological processes such as protein complex assembly, and involved in the phagocytic Fcγ receptor signaling pathways and receptor family signaling pathways. The protein-protein interaction networks showed that TNF was the core protein node. The microRNA-gene regulatory network showed that hsa-mir-124-3p interacted with differentially expressed genes; TNF, KYNU, RCAN1 and other related genes all interacted with environmental factors. The gene expression of adipose tissue CD14+ cells are significantly changed in obese T2DM patients. TNF may play an important role in the process of obesity affecting the immune status of T2DM patients. Multiple microRNAs, transcription factors, and environmental factors also play a role in the above process. This study provides new material and new ideas for further exploration of the impact of obesity on T2DM patients.