Abstract
We developed a rapid and accurate method for quantifying gaseous phase odorants using headspace solid-phase microextraction (HS-SPME) in conjunction with GC-MS and used our system to quantify alkylpyrazine analogs in the Y-maze. Rapid extraction of volatile compounds in the vapor phase achieved accurate quantitative analysis of gaseous alkylpyrazine analogs at several locations in the Y-maze. We also used a series of three SPME fibers to quantify changes in the concentration over time. We conducted a behavioral test of mice in response to these alkylpyrazines and identified a positive relationship between the rate of increase in gaseous concentration and the avoidance rate induced. Our results demonstrate that the Y-maze is a simple but reliable apparatus for behavioral studies of olfaction. The HS-SPME fast extraction method can quantify how gaseous concentrations of alkylpyrazines change over time, and the time-dependent increase of alkylpyrazine concentration is correlated with induction of aversive behavior in mice.
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