Changes in extracellular potassium and calcium concentration and neural activity during prolonged electrical stimulation of the cat cerebral cortex at defined charge densities

Abstract

In cats anesthetized with nitrous oxide and halothane, ion-selective microelectrodes were used to monitor changes in the concentration of potassium [K +] 0 and calcium [Ca 2+] 0 in the extracellular compartment of the cerebral cortex during as long as 4 h of continuous stimulation of the cortical surface. At stimulus charge densities shown to induce only minimal localized histologic changes [20 μC/cm 2·ph at 50 pulses per second (pps)], [K +] 0 at a depth of about 750 μm underwent only a transient increase at the beginning of stimulation, followed by a rapid return to the prestimulus concentration. [Ca 2+] 0 was unaffected. At a higher charge density (100 μC/cm 2·ph at 20 pps) there was a rapid transient increase in [K +] 0, followed by a more gradual return to a plateau about 1 m m above the prestimulus value. [Ca 2+] 0 usually underwent an initial increase followed by a slow decrease to a plateau value above 0.5 m m. At a charge density of 100 μC/cm 2·ph and 50 pps (shown in histological studies to induce significant neural damage), [Ca 2+] 0 slowly decreased to near or below 0.5 m m in the middle layers of the cortex. After 30 to 40 min of stimulation, [K +] 0 underwent episodic fluctuations about a plateau value 0.5 to 1 m m above the prestimulus concentration. Simultaneous recordings of the compound action potential in the ipsilateral pyramidal tract indicated that these fluctuations were due to local changes in the excitability of intracortical circuitry conditioned by the intense stimulation. The results have implications for the possible interrelation of the changes in extracellular ionic concentrations and the early stages of stimulation-induced neural damage.