Changes in expression of CXCR3 in regulatory T cells in renal tissues of mice with renal ischemia-reperfusion injury

Abstract

Objective To evaluate the changes in the expression of CXCR3 in regulatory T cells （Tregs） in the renal tissues of mice with renal ischemia-reperfusion （I/R） injury .Methods Forty-eight SPF male C57BL/6J mice ,aged 8-12 yr ,weighing 20-25 g ,were randomly divided into 3 groups （ n=16 each ） using a random number table：sham operation group （group S） ,group I/R and CD25 monoclonal antibody PC61 group （group P） . Bilateral kidneys were exposed and their pedicles were occluded for 45 min with atraumatic mini-clamp followed by 72 h reperfusion .PC61 250 μg was injected intraperitoneally at 24 h before the model was established .Blood samples were collected from the inferior vena cava at 24 and 72 h of reperfusion （T1 ,2 ） for determination of serum blood urea nitrogen （BUN） and creatinine （Cr） concentrations .Bilateral kidneys were obtained for determination of CD4＋ CD25＋ Foxp3＋ Treg count and CXCR3＋ CD4＋ CD25＋ Foxp3＋ Treg count in renal tissues and the pathological changes of the kidney were scored .Results Compared with group S , the serum BUN and Cr concentrations and pathological scores were significantly increased at T1 ,2 in I/R and P groups ,and the number of CD4＋ CD25＋ Foxp3＋ Treg and CXCR3＋ CD4＋ CD25＋ Foxp3＋ Treg was increased at T2 in I/R group （ P〈0.05） .Compared with group I/R ,the serum BUN and Cr concentrations and pathological scores were significantly increased at T2 ,and the number of CD4＋ CD25＋ Foxp3＋ Treg and CXCR3＋ CD4＋ CD25＋ Foxp3＋ Treg was＆nbsp;decreased at T2 in P group （ P〈0.05 ） .Conclusion Up-regulation of CXCR3 is helpful in migration of Tregs into the renal tissues of mice with renal I/R injury . Key words: Receptors,CXCR3; T-lymphocytes,regulatory; Kidney; Reperfusion injury