Changes in ether-linked phospholipids in rat kidney by dietary alpha-linolenic acid in vivo.

Abstract

We investigated the effects of perilla oil containing a high level of alpha-linolenic acid on in vivo phospholipid metabolism, particularly three subclasses of choline glycerophospholipids (CGP) and ethanolamine glycerophospholipids (EGP), in rat kidney. After three weeks of feeding, a significantly lower proportion (by 35%) of the alkylacyl subclass of CGP was found in the perilla oil, as compared to corn oil-fed animals. The alkylacyl species of EGP was also higher in the perilla oil than in the corn oil-fed animals. These alterations were accompanied by a remarkably lower proportion of arachidonic acid and a higher level of eicosapentaenoic acid (EPA) in all six subclasses of CGP and EGP in the perilla oil-fed animals. The levels of linoleic acid were even higher in the diacyl subclasses of CGP and EGP in the perilla oil group, suggesting that desaturase and elongase enzymes prefer n-3 to n-6 fatty acids as substrates for diacyl species. These data are useful in defining the effects of alpha-linolenic acid on the biosynthesis of renal phospholipids and on the replacement of n-6 with n-3 fatty acids in the six CGP and EGP subclasses.