Changes in erythrocyte ATPase activity under different pathological conditions.

Ali A Kherd,Etimad A Huwait,Said S Moselhy,Nawal Helmi,Taha A Kumosani,Soonham S Yaghmoor,Shareefa A Al-Ghamdi,Maryam A Al-Ghamdi,Qari M,Alaama Nabil,Khadijah Saeed Balamash

doi:10.4314/ahs.v17i4.31

Abstract

Studies have shown that Na+-K+ ATPase activity was altered in disrupted red blood cell membranes and this enzyme is believed to be the site of active transport of Na+ and K+ in intact red blood cells. The enzyme is often referred to as Na+-K+ pump because it pumps Na+ out and K+ into the cell against gradients with the concomitant hydrolysis of intracellular ATP. The aim of this study was to find out the possibility of using Na+-K+-ATPase activity as a biomarker for the diagnosis of individuals with different physiological conditions. The activity of Na+-K+ ATPase was determined in blood samples collected from different pathological and physiological conditions such as pregnancy, smoking, diabetes and renal dysfunction compared with healthy subjects matched for age and sex. The Na+-K+ ATPase activity in pregnancy (0.094 ± 0.0051 µM Pi/min. mg protein), smoking (0.064 ± 0.0011 µM), diabetes (0.047 µM 0.002 µM) and kidney disease (0.069 ± 0.0014 µM) was higher compared to the measurements in healthy individuals (0.0081 ± 0.0031 µM). Na+-K+ATPase specific activity is a biomarker for the diagnosis of individuals with different physiological diseases.

Highlights

Studies have shown that Na+-K+-ATPase activity was African Health Sciences@ 2017 Kherd et al; licensee African Health Sciences
Background: Studies have shown that Na+-K+ ATPase activity was altered in disrupted red blood cell membranes and this enzyme is believed to be the site of active transport of Na+ and K+ in intact red blood cells
Materials and methods: The activity of Na+-K+ ATPase was determined in blood samples collected from different pathological and physiological conditions such as pregnancy, smoking, diabetes and renal dysfunction compared with healthy subjects matched for age and sex

Summary

Introduction

Studies have shown that Na+-K+-ATPase activity was African Health Sciences@ 2017 Kherd et al; licensee African Health Sciences. Studies have shown that Na+-K+-ATPase activity was African Health Sciences. African Health Sciences Vol 17 Issue 4, December, 2017 the phosphorylation of the transport protein itself This phosphorylation results in a change in the conformation of the protein that, in turn, plays a part in the translocation of one or more substrate molecules across the membrane[4]. Intra-erythrocyte free Ca-2+ concentration in young individuals was markedly higher than that in adults[3]. Erythrocyte Na+, K+-ATPase activity was normal in pregnant women, suggesting that the peripheral metabolic status during pregnancy was normal. Increases in both the number and function of the pump may be influenced by factors other than thyroid function[7]. Materials and methods: The activity of Na+-K+ ATPase was determined in blood samples collected from different pathological and physiological conditions such as pregnancy, smoking, diabetes and renal dysfunction compared with healthy subjects matched for age and sex

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