Changes in Epigenetic Patterns Related to DNA Replication in Vicia faba Root Meristem Cells under Cadmium-Induced Stress Conditions.

Aneta Żabka,Justyna Teresa Polit,Natalia Gocek,Konrad Winnicki,Paweł Szczeblewski,Tomasz Laskowski

doi:10.3390/cells10123409

Aneta Żabka, Justyna Teresa Polit + Show 4 more

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https://doi.org/10.3390/cells10123409

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Journal: Cells	Publication Date: Dec 3, 2021
Citations: 5	License type: CC BY 4.0

Affiliation: University of Łódź, Gdańsk University of Technology

Abstract

Experiments on Vicia faba root meristem cells exposed to 150 µM cadmium chloride (CdCl2) were undertaken to analyse epigenetic changes, mainly with respect to DNA replication stress. Histone modifications examined by means of immunofluorescence labeling included: (1) acetylation of histone H3 on lysine 56 (H3K56Ac), involved in transcription, S phase, and response to DNA damage during DNA biosynthesis; (2) dimethylation of histone H3 on lysine 79 (H3K79Me2), correlated with the replication initiation; (3) phosphorylation of histone H3 on threonine 45 (H3T45Ph), engaged in DNA synthesis and apoptosis. Moreover, immunostaining using specific antibodies against 5-MetC-modified DNA was used to determine the level of DNA methylation. A significant decrease in the level of H3K79Me2, noted in all phases of the CdCl2-treated interphase cell nuclei, was found to correspond with: (1) an increase in the mean number of intranuclear foci of H3K56Ac histones (observed mainly in S-phase), (2) a plethora of nuclear and nucleolar labeling patterns (combined with a general decrease in H3T45Ph), and (3) a decrease in DNA methylation. All these changes correlate well with a general viewpoint that DNA modifications and post-translational histone modifications play an important role in gene expression and plant development under cadmium-induced stress conditions.

Highlights

Due to a sedentary lifestyle, plants are constantly exposed to a variety of environmental stresses which largely hinder their proper development
After the application of CdCl2, a slight shift to the right of the maximum values for the G1and G2-phase cell cycle subpopulations was observed. This result is probably due to the increased level of chromatin condensation. It can be noted (Figure 1D) that an increase in the number of the G1 phase cells in CdCl2-treated roots (30.1% compared to 26.1% in the control) is accompanied by a slight increase in the number of cells in the S (44.8% compared to 41.7%) and a more significant rise in the number of G2 phase cells (25.1% compared to 32.2%; Figure 1B–D)
It has been shown that: (i) epigenetic marks are used to change signaling pathways which alter cellular metabolism and, eventually, allow plants to protect themselves from possible DNA damage caused by Heavy metals (HMs); (ii) high levels of methylation can protect DNA from endonuclease cleavage, which increases the resistance of cells to HMs; (iii) epigenetic changes serve to regulate genes responding to stress factors [3]

Summary

Introduction

Due to a sedentary lifestyle, plants are constantly exposed to a variety of environmental stresses which largely hinder their proper development. In order to prevent stressful situations, plants have developed complicated and complex mechanisms for stress detection and defense reactions [4]. They activate various signal transduction pathways to allow the expression of stress-responsive genes, which induce changes at the morphological, physiological, and biochemical levels that adapt plants to adverse environmental conditions [5,6]. The mechanism of genome-wide DNA duplication precisely carries out its functions despite numerous obstacles of intracellular and extracellular origin, many of which can lead to “replication stress” (RS).

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