Abstract
Enzyme and metabolite content of effuent perfusate has been investigated. One kidney was removed from dogs and preserved by Collin's method. The animals were then bled to a mean arterial blood pressure of 5.26 KPa (40 mm Hg) and maintained at this pressure for 60 min after which the other kidney was removed and preserved in a similar way. 10-ml samples of effluent perfusate were taken immediately, and after 30 min, 1, 2, 3, 4, 6, 8, 24, 48 and 72 h. Acid phosphatase, arylsulphatase "A", ss-glucuronidase, LDH, leucin-aminopeptidase, lactic acid and pyruvic acid were determined by fluorometric methods. It was found that the activity of the enzymes rose parallel with the duration of preservation, the rise was especially great at 48 and 72 h. Activity of LDH and arylsulphatase "A" was significantly higher even at the beginning of preservation in the group of kidneys exposed in vivo to hypotension. This significant difference was present at 24, 48 und 72 h in the activity of all enzymes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.