Abstract
Haploid tobacco tissues cultured on Murashige and Skoog (MS) medium supplemented with 0.3 mg/l indole-3-acetic acid (IAA) and 1% sucrose remained undifferentiated. Increasing sucrose level (3%) at the same IAA concentration induced shoot differentiation in 9 days. Further increase in sucrose level (6%) resulted in root differentiation on day 12. Total specific activity of peroxidase, IAA oxidase, malate dehydrogenase (MDH) and phenylalanine ammonia-lyase (PAL) exhibited definite development variations among the three programmes. The number of the anodic and cathodic isoperoxidase bands changed in each case with time. Shoot formation was characterized by the synthesis of anodic peroxidases: AS 1 ( Rm=0.41), AS 2 ( Rm=0.44) and AS 3 ( Rm=0.46), all three being synthesized prior to visual manifestation of shoots. Likewise, root differentiation was heralded in advance by synthesis of one anodic isoperoxidase AR ( Rm=0.30). Cathodic isoperoxidases did not show any subtle correlation with shoot or root formation.
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