Changes in Enzymatic content of Rice var. ADT 36 as influenced by bioinoculant, plant activator and sheath blight pathogen inoculation

Abstract

The Pot culture studies were undertaken to investigate the changes of enzymatic compound in rice as influenced by Bioinoculant Pseudomonas fluorescens, plant activator salicylic acid and Sheath blight pathogen Rhizoctonia solani inoculation. Among the various treatments seed treatment with P. fluorescens (Pf) @ 10 g/kg of seeds along with foliar application of plant activator Salicylic acid (SA) @ 50 ppm on 15 Days After transplanting (DAT) recorded the minimum disease incidence followed by foliar application of plant activator Salicylic acid @ 50 ppm on 15 DAT and 30 DAT which were statistically at par. Foliar application of Pf @ 1% on 30 DAT was the least effective when compared to fungicide, Hexaconazole 5 SC @ 0.2% and control treatments. Also, the results exploited that the same treatment increased activity of Ascorbic acid oxidase, PAL, PPO and PO activity due to treatment with combined application of resistance inducing chemical, bioinoculant and pathogen alone inoculated control when compared to control treatments. The activity of PO, Ascorbic acid oxidase and PAL increased up to 14th day of sampling and then decreased in plants treated with the combination treatment consisting of Pf, SA and challenge inoculated with the pathogen whereas the activity of Polyphenol oxidase showed increase up to the maximum period of observation (up to 21st day). The cost benefit ratio 1: 2.63 proved the worthiness of the new treatment combination developed in this study.