Changes in energy reserves and responses of some biomarkers in freshwater mussels exposed to metal-oxide nanoparticles

Abstract

In this study, responses of various biomarkers in the digestive gland and foot muscle of freshwater mussels (Unio tigridis) were investigated following exposure to Al2O3, CuO and TiO2 nanoparticles (NPs) for 14 days at different concentrations (0, 1, 3 and 9 mg NP/L). Mussels were fed on unicellular algae (Chlorella vulgaris) cultured in the laboratory. NP exposures caused significant increases (p < 0.05) in the levels of total glutathione (GSH), reduced-glutathione (rGSH), oxidized-glutathione (GSSG) and malondialdehyde (MDA) in the digestive gland. Oppositely, there were significant (p < 0.05) decreases in acetylcholinesterase activity in the foot muscles. Total energy reserves of the digestive gland and foot muscle significantly (p < 0.05) decreased, but only at the highest NP exposures. Nevertheless, NP exposures did not alter (p > 0.05) the algae filtering capacity of mussels. This study demonstrated that the biomarkers belonging to different metabolic systems responded to NP exposures, suggesting their usage in the monitoring studies for freshwater systems.