Abstract
The mechanism of decrease in cell electrophoretic mobility after X-irradiation was tentatively envisaged as change of cell surface conformation, based upon the following results: (1) Electrophoretic mobility was mainly dependent on the negative charge of sialic acid, chondroitin sulfates, and hyaluronic acid of the cell surface. Radioactive labels incorporated into these acidic sugars were not affected by X-irradiation at the time when the electrophoretic mobility showed minimum value. (2) Sulfhydryl-blocking agents (p-chloromercuribenzoic acid, N-ethylmaleimide and iodoacetamide) at concentrations of $1\times 10^{-8}\sim 10^{-6} M$ completely prevented the reduction in electrophoretic mobility after irradiation. (3) Addition of phytohemagglutinin-M or Concanavalin A to cell cultures before or after irradiation also stopped the decrease in mobility. (4) The rates of decrease and recovery in electrophoretic mobility of X-irradiated cells were not substantially affected by a 9°C temper...
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