Changes in electricity production and microbial community evolution in constructed wetland-microbial fuel cell exposed to wastewater containing Pb(II)

Abstract

Two constructed wetland microbial fuel cell (CW-MFC) devices, experimental group (EG, with 5 mg/L Pb(II) addition) and control group (CG) were built to explore the changes in power generation, wastewater purification and microbial community structure under Pb(II) stress. The voltage of EG (343.16 ± 12.14 mV) was significantly higher (p < 0.01) than that of CG (295.49 ± 13.91 mV), and the highest power density of the EG and CG were 7.432 mW·m−2 and 3.873 mW·m−2, respectively. There was no significant difference in the removal of common pollutants between these groups except for the NH4+-N removal efficiency, which was probably caused by the inhibition of the bioactivity of Comamonas (AOB) in the anode of the experimental group by Pb(II). Pb(II) was effectively removed by CW-MFC (84.86 ± 3%), and the abundant amount of fulvic acid-like matter in the extracellular polymeric substance (EPS) of the EG contributed to its removal. The presence of Pb(II) had a negative effect on both microbial community diversity and species richness. The abundance of a lead resistance gene, pbrT, decreased with long-term Pb(II) pressure. This is evidence of microbial adaptation to Pb(II).