Abstract
Studies of the electrical surface properties of biological cells have provided fundamental knowledge about the cell surface. The change in biological functions of cells may affect the surface properties and can be detected by electrokinetic measurements. The surface density of fibroblasts and breast cancer cells (MDA-MB-231 and MCF-7) as a function of pH was measured by electrophoresis. The interaction between solution ions and the breast cancer cell or fibroblast surface was described by a four-component equilibrium model. The agreement between the experimental and theoretical charge variation curves of the breast cancer cells and fibroblasts was good at pH 2.5–9. The extent of fibroblast and breast cancer cell lipid peroxidation was estimated by HPLC measurement of the malondialdehyde level. The acid (C TA) and basic (C TB) functional group concentrations and the average association constant with hydroxyl (K BOH) ions values of the breast cancer cell membranes were higher than in normal cells, while the average association constant with hydrogen (K AH) value was smaller. The level of lipid peroxidation products was higher in breast cancer cells than in normal cells.
Highlights
The electric charge of cell membranes of mammals is negative at physiological pH (Benga and Holmes 1984)
The interaction between solution ions and the breast cancer cell or fibroblast surface was described by a four-component equilibrium model
Determining the electric charge of the membrane as a function of environmental pH, acid (CTA) and basic (CTB) functional group concentrations and their average association constants with hydrogen (KAH) or hydroxyl (KBOH) ions allows monitoring of changes caused by cancer transformation (Dobrzynska et al 2006)
Summary
The electric charge of cell membranes of mammals is negative at physiological pH (Benga and Holmes 1984). An essential property of the electric double layer is its electric charge, which can be altered by various xenobiotics or by metabolic transformations. For this reason, studies of electric charge can furnish much information on the equilibrium existing within the membrane and between the membrane and its environment, both in physiological and in nonphysiological conditions (Szachowicz-Petelska et al 2012; Gennis 1989; Dołowy 1984). Determining the electric charge of the membrane as a function of environmental pH, acid (CTA) and basic (CTB) functional group concentrations and their average association constants with hydrogen (KAH) or hydroxyl (KBOH) ions allows monitoring of changes caused by cancer transformation (Dobrzynska et al 2006)
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