Abstract
Summary Histone and non-histone proteins were extracted from cells during the early stage of embryogenesis in a carrot suspension culture and were analysed by electrophoresis. Seven major bands of protein were detected in the non-histon protein, there being no remarkable differences between cells cultured in the embryogenetic medium and in the non-embryogenetic medium. For embryo induced cells, a lower ratio of histone H1 to total histone, and a higher ratio of H2+H3 to H4 were observed than for non-induced cells. The relationship between events during embryogenesis and changes in chromosomal proteins is discussed.
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