Abstract
Understanding plant’s response mechanisms against pathogenesis is fundamental for the development of resistant crop varieties and more productive agriculture. In this regard, “omic” approaches are heralded as valuable technologies. In this work, combining isobaric tags for relative and absolute quantification (iTRAQ) technology with mass spectrometry, the proteomes from leaves of Brassica oleracea plants infected with Xanthomonas campestris pv. campestris (Xcc), and control plants at two different post-infection times were compared. Stronger proteomic changes were obtained at 12 days post-infection in comparison with 3 days. The responses observed involved different cell processes, from primary metabolism, such as photosynthesis or photorespiration, to other complex processes such as redox homeostasis, hormone signaling, or defense mechanisms. Most of the proteins decreased in the earlier response were involved in energetic metabolism, whereas later response was characterized by a recovery of primary metabolism. Furthermore, our results indicated that proteolysis machinery and reactive oxygen species (ROS) homeostasis could be key processes during this plant–pathogen interaction. Current data provide new insights into molecular mechanisms that may be involved in defense responses of B. oleracea to Xcc.
Highlights
During their vital cycle, plants are exposed to several unfavorable growing conditions, which often cause significant damages or even plant death
We carried out a proteomic approach by combining isobaric tags for relative and absolute quantification (iTRAQ) labeling, LC separation, and MS approach to investigate the protein changes occurring in the complete set of proteins—the “proteome”—of B. oleracea plants infected with Xanthomonas campestris pv. campestris (Xcc) race 1 at different time infection points
It is worth mentioning that we tried to identify pathogen proteins among the proteomic results by using several bacterial databases, but no proteins with bacterial origin was found in this study
Summary
Plants are exposed to several unfavorable growing conditions, which often cause significant damages or even plant death. Proteomic data of B. oleracea–Xcc interaction have been reported in several studies (Santos et al, 2017, 2019; Ribeiro et al, 2018; Zhang et al, 2020; Islam et al, 2021) by using different approach as label-free shotgun 2D-nanoUPLC/MSE and liquid chromatography–tandem mass spectrometry (LC– MS/MS) (Santos et al, 2017; Islam et al, 2021) Technologies, such as isobaric tags for relative and absolute quantification (iTRAQ), have been developed to overcome the problems derived from gel-based approaches. We carried out a proteomic approach by combining iTRAQ labeling, LC separation, and MS approach to investigate the protein changes occurring in the complete set of proteins—the “proteome”—of B. oleracea plants infected with Xcc race 1 at different time infection points
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