Changes in arbuscular mycorrhizal fungal attributes along a chronosequence of black locust (Robinia pseudoacacia) plantations can be attributed to the plantation-induced variation in soil properties

Abstract

Arbuscular mycorrhizal (AM) fungi form symbioses with the majority of terrestrial plant species, and their community influences many important ecosystem processes, including ecological succession. Understanding the successional changes of AM fungal communities in afforested zones over time is of primary interest in forest ecology. Black locust (Robinia pseudoacacia) has been widely planted on the Loess Plateau of China to prevent soil erosion. We sampled fine roots and rhizosphere soils in black locust plantations consisting of stands of 0, 11, 23, 35 and 46years of age to measure soil properties, AM fungal colonization level, and spore density and to describe the composition of AM fungal communities in roots and soils using 454 sequencing. With increasing stand age, AM fungal spore density and soil NO3-N and available K contents increased, dehydrogenase and alkaline phosphatase activities decreased, and soil catalase activity and the level of root colonization by arbuscules and hyphae first increased and then decreased. Roots and soils hosted different AM fungal diversity and communities. In soils, AM fungal diversity and community composition did not vary with stand age. In roots, the relative abundance of Claroideoglomus, together with Chao1 richness and OTU richness, peaked at the intermediate stage (35years) and then declined, and the relative abundance of Glomus decreased linearly with tree age, whereas the relative abundance of the dominant genus Rhizophagus did not vary with stand age. Soil available K and NO3-N largely explained the shift in the structure of the root-colonizing AM fungal community along the chronosequence. Soil enzyme activities were also associated with changes in AM fungal spore abundance and root colonization level. All the results presented here suggest that the successional changes in AM fungal communities in black locust plantations occurring over time can largely be attributed to plantation-induced changes in soil nutrient levels.