Abstract
Background: Adenosine deaminase (ADA) and 5'-nucleotidase (5'-NT) play a crucial role in adenosine metabolism in healthy individuals. Adenosine is an inflammatory mediator of asthma. Changes in adenosine metabolism and role of ADA and 5'-NT in regulating adenosine level in asthmatics and correlation of these changes with severity of asthma are not clearly understood. Methods: In this study, we screened 5217 patients, of which 2416 were diagnosed with asthma. Further, of 2416 asthmatics, only 45 patients who strictly fulfilled the selection criteria were enrolled in the study. The patients were classified into mild, moderate and severe persistent groups; each group consisted of fifteen patients. Fifteen healthy subjects served as controls. Adenosine levels and activities of 5'-NT, total ADA, ADA1 and ADA2 in serum, lymphocytes and erythrocytes were determined. The data were analysed statistically and p vice versa.
Highlights
Bronchial asthma is one of the most common chronic diseases globally and currently affects nearly 300 million people [1]
Since studies measuring activities of these enzymes and correlating it with adenosine levels in bronchial asthma patients are lacking, we proposed the present study to examine the activities of adenosine deaminase, its isoenzymes, 5ʹ-nucleotidase and adenosine levels in serum, erythrocytes and lymphocytes to understand the metabolism of adenosine in bronchial asthma
The present study clearly demonstrates that the adenosine levels are raised in serum, lymphocytes and erythrocytes in bronchial asthma patients and correlation of this increase in adenosine with increase in airway obstruction provides evidence in favour of adenosine for its role as a crucial inflammatory mediator in asthma
Summary
Bronchial asthma is one of the most common chronic diseases globally and currently affects nearly 300 million people [1]. There is good evidence that the specific pattern of airway inflammation in asthma is associated with airway hyperresposiveness that is further correlated with variable airflow obstruction [4] Several cell types such as lymphocytes, mast cells, macrophages, eosinophils, neutrophils and epithelial cells produce inflammatory changes by release of various mediators like adenosine, histamine, kinin, leukotrienes, prostaglandins, PAF, chemokines and cytokines which interact in a complex way to produce airway inflammation [4]. Changes in adenosine metabolism and role of ADA and 5ʹ-NT in regulating adenosine level in asthmatics and correlation of these changes with severity of asthma are not clearly understood. A significant reciprocal correlation existed between adenosine levels in serum, lymphocytes and erythrocytes of asthmatics and FEV1%. The activities of total ADA, ADA1 and ADA2 were decreased in serum and lymphocytes of moderate and severe persistent asthmatics and a positive correlation existed between total ADA and FEV1%.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
