Abstract
Changes in IgG mRNA half-life, transcription and nuclear and cytoplasmic abundance were studied in two cell lines which contain an identical Ig γ 2a heavy chain but which differ in its expression. The A20.2J mouse lymphoma expresses about equal amounts of Ig γ 2a secretory- and membrane-specific mRNAs whereas in the AXJ hybrids, resulting from the fusion of A20.2J with the J558L myeloma, the secretory-specific form dominates. Further evidence of dominance of the myeloma phenotype was seen in the large changes in mRNA abundance and nuclear accumulation as well as in a small increase in Ig γ 2a mRNA half-lives for both secretory and membrane forms. Contributing to the observed > 100-fold increase in the ratio of secretory vs membrane forms of the Ig γ 2a heavy chain in the AXJ hybrids are both a 10-fold decrease in the production of the membrane form by post-transcriptional RNA processing events and a ≈ 6–7-fold decrease in the nuclear to cytoplasmic ratio for the Ig secretory γ 2a and κ light chain RNAs. Differential RNA accumulation in the nucleus in the lymphma cell therefore contributes to the differential expression of Ig secretory mRNA.
Published Version
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