Changes and significance of plasma cardiotrophin-1 in children with heart failure

Abstract

Objective To explore the changes and significance of plasma cardiotrophin-1(CT-1) in children with heart failure. Methods Forty children with heart failure(NYHA II, n=14; NYHA Ⅲ, n=16; NYHA Ⅳ, n=10) were chosen as observation group, and 20 healthy children without heart failure were taken as healthy control group.Plasma CT-1 was measured by a double antibody sandwich enzyme-linked immunosorbent assay(ELISA). N terminal probrain natriuretic peptide(NT-pro BNP) concentration was tested by Pu Rui fluorescent dry quantitative analyzer.Left ventricular ejection fraction(LVEF) was evaluated by GE Vivid 7 doppler echocardiography and cardiac function assessed by modified ROSS score.Plasma CT-1 level, NT-pro BNP and LVEF were compared between the 2 groups.The correlation of plasma CT-1 level with NT-pro BNP and LVEF were analyzed in patients with different degrees of heart failure. Results Plasma levels of CT-1 and NT-pro BNP were significantly higher in children with heart failure than those in healthy control group(P<0.01), and with progression of heart failure, the levels of CT-1 and NT-pro BNP progressively increased in heart failure children.Plasma CT-1 level in different NYHA had statistical significance(F=55.5, P<0.01). Plasma CT-1 level was positive correlated with NT-pro BNP and modified ROSS score(r=0.787, 0.848, all P<0.01), and negative associated with LVEF(r=-0.66, P<0.01), respectively, in heart failure children. Conclusions Plasma CT-1 level is significantly elevated in heart failure children.There are good correlation among CT-1, NT-pro BNP and LVEF.Plasma CT-1 is a reliable marker of reflecting the severity of heart failure, combined with NT-pro BNP detection helps to improve the diagnostic accuracy of heart failure in children. Key words: Cardiotrophin-1; N terminal probrain natriuretic peptide; Heart failure; Child