Changes and significance of B cell specific monoclonal murine leukemia virus integration site-1 and helper T cell-related cytokine in children with immune thrombocytopenia

Abstract

Objective To analyze the functional changes and the clinical significance of B cell specific monoclonal murine leukemia virus integration site-1(Bmi-1 ) and Th1/Th2 cells in children with newly diagnosed immune thrombocytopenia(ITP) by testing the mRNA expressions of Bmi-1, helper T cell-related cytokine interferon(IFN)-γ and interleukin(IL)-4 in children with newly diagnosed ITP. Methods Thirty-six cases of patients with newly diagnosed ITP in the experimental group came from the inpatient and outpatient children admitted to the Department of Pediatrics of the First Affiliated Hospital of Xinxiang Medical University from April to December 2013.In the control group, 26 cases of children requiring selective operation were admitted to the Department of Pediatric Surgery during the same period.The mRNA expressions of Bmi-1, IFN-γ and IL-4 in the peripheral blood lymphocytes were detected by means of the reverse transcription-polymerase chain reaction(RT-PCR) method, and were analyzed and compared by t test and linear correlation analysis. Results (1) The mRNA expressions of Bmi-1, IFN-γ and IL-4 in peripheral blood lymphocytes in the experimental group were 2.63±0.54, 3.84±0.43 and 1.44±0.39, respectively; while the mRNA expressions of Bmi-1, IFN-γ and IL-4 in the peripheral blood lymphocytes in the control group were 3.91±0.92, 2.88±0.57 and 1.87±0.34, respectively.The levels of IFN-γ of the experimental group were significantly higher than those of the control group (P 0.05). Conclusions Bmi-1 may be involved in the pathogenesis of ITP by regulating Th cell, and Th cell dysfunction may occur in the children with ITP, and the disproportion between Th1 and Th2 may be due to the advantages of Th1. Key words: B cell specific moloney murine leukemia virus integration site-1; Helper T cells; Immune thrombocytopenia; Child